polystyrene 48 well plates  (Greiner Bio)

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Biofilm mass of S. aureus strains (n=26) cultured on polystyrene (PS) surface and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). Each box displays the interquartile range (IQR; 25th to 75th percentiles), with the bold horizontal line indicating the median. Whiskers extend to the most extreme data points within 1.5 × IQR from the lower and upper quartiles. Technical repetitions are shown as dots. Differences were statistically significant, p ≤ 0.0001. Dunn’s test was performed. Adjusted p value includes Bonferroni correction (Supplementary Table S3).

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Biofilm viable cell number of S. aureus strains (n=10) cultured on different surfaces: polystyrene (PS) or biocellulose (BC), and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). (A) Dividing condition: medium and surface. (B) Dividing condition: medium. (C) Dividing condition: surface. Each box displays the interquartile range (IQR; 25th to 75th percentiles), with the bold horizontal line indicating the median. Whiskers extend to the most extreme data points within 1.5 × IQR from the lower and upper quartiles. Technical repetitions are shown as dots. CFU/mL-colony-forming unit/mL.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet:

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Activity Assay, Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Biofilm metabolic activity of S. aureus strains (n=26) cultured on different surfaces: polystyrene (PS) or biocellulose (BC), and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). (A) Dividing condition: medium and surface. (B) Dividing condition: medium. (C) Dividing condition: surface. Each box displays the interquartile range (IQR; 25th to 75th percentiles), with the bold horizontal line indicating the median. Whiskers extend to the most extreme data points within 1.5 × IQR from the lower and upper quartiles. Technical repetitions are shown as dots.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Activity Assay, Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Scatter plots of correlations of S. aureus strains’ (n=26) biofilm mass and biofilm metabolic activity. Biofilms were cultured on polystyrene (PS) and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). (A) Dividing condition: medium. (B) Dividing condition: surface. The points denote the means for each strain. Data fitted on a linear trend line. The equation for the line of best fit and R 2 -coefficient of determination are presented in the top left corners of the respective panels.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Activity Assay, Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Scatter plots of correlations of S. aureus strains’ (n=10) biofilm metabolic activity and biofilm viable cell number (CFU/mL-colony-forming unit/mL). Biofilms were cultured on different surfaces: polystyrene (PS) or biocellulose (BC), and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). (A) Dividing condition: medium and surface. (B) Dividing condition: medium. (C) Dividing condition: surface. The points denote the means for each strain. Data fitted on a linear trend line. The equation for the line of best fit and R 2 -coefficient of determination are presented in the top left corners of the respective panels.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Activity Assay, Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Antibiofilm activity of rosemary essential oil (REO) or thyme essential oil (TEO) against S. aureus strains (n=10) cultured on different surfaces: polystyrene (PS) or biocellulose (BC) and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM), assessed with a dilution method. (A, B) Dividing condition: medium and surface. (C, D) Dividing condition: medium. (E, F) Dividing condition: surface. The points denote the mean value, and the error lines denote the standard error of measurement.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Activity Assay, Cell Culture, In Vitro

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Microscopic visualization of S. aureus biofilms (n=2) grown on polystyrene in tryptic soy broth (TSB) or in in vitro wound milieu medium (IVWM) and treated with volatile fractions of rosemary essential oil (REO) or thyme essential oil (TEO). C+ is a growth control setting, where biofilms were non-exposed to antimicrobials. (A) S3 strain in TSB. (B) S3 strain in IVWM. (C) S4 strain in TSB. (D) S4 strain in IVWM. All biofilms were stained using the Filmtracer™ LIVE/DEAD™ Biofilm Viability Kit to assess cell viability. Green fluorescence indicates live cells with intact membranes, while red fluorescence marks cells with compromised membrane integrity. Images represent a full-well of a 24-well plate tiling acquired using a 4× objective and ImageJ Software. Microscope Lumascope 620. The well diameter was 15 mm.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: In Vitro, Control, Staining, Fluorescence, Membrane, Software, Microscopy

Journal: bioRxiv

Article Title: Establishing Essential Oil Stewardship Through the Case of Rosemary and Thyme Oils Against Staphylococcus aureus

doi: 10.1101/2025.07.16.665039

Figure Lengend Snippet: Scatter plots of correlations of S. aureus strains’ (n=10) biofilm mass and biofilm viable cell number (CFU/mL-colony-forming unit/mL). Biofilms were cultured on polystyrene (PS) and in different media: tryptic soy broth (TSB) or in in vitro wound milieu (IVWM). (A) Dividing condition: medium. (B) Dividing condition: surface. The points denote the means for each strain. Data fitted on a linear trend line. The equation for the line of best fit and R 2 -coefficient of determination are presented in the top left corners of the respective panels.

Article Snippet: 48-well polystyrene plates with the wells’ diameters of 11 mm (Wuxi Nest Biotechnology, China) served as the PS surface, and 2% (w/v) bacteriological lab agar (Biomaxima, Poland) was used to prepare agar discs (with a diameter of 11 mm).

Techniques: Cell Culture, In Vitro

Journal: Infection and Immunity

Article Title: Structural basis of aggregative adherence fimbriae II interactions with sialic acid, mucin, and human intestinal cells

doi: 10.1128/iai.00483-24

Figure Lengend Snippet: EAEC binds to mucin and fibronectin, and adherence to both substrates is AAF-dependent. Bacterial strains were incubated in polystyrene plates precoated with bovine submaxillary mucin or human fibronectin for 90 minutes at 37°C. Plates were then washed three times with PBS and treated with 0.1% Triton X-100/PBS, and adherent bacteria were enumerated by dilution and plating. Two-way ANOVA was performed on log-transformed data followed by Bonferroni’s test for multiple comparisons. Significance indicated as compared to BSA control. * P ≤ 0.05, **** P ≤ 0.0001.

Article Snippet: Briefly, bacterial strains were grown to an OD 600 of 1.0 in LB at 37°C with shaking and then diluted 1:50 into DMEM high glucose with 2% arabinose and 100 μg/mL carbenicillin in 48-well polystyrene plates (Corning).

Techniques: Incubation, Bacteria, Transformation Assay, Control

Journal: Infection and Immunity

Article Title: Structural basis of aggregative adherence fimbriae II interactions with sialic acid, mucin, and human intestinal cells

doi: 10.1128/iai.00483-24

Figure Lengend Snippet: Identification of AafA residues that contribute to binding to bovine submaxillary mucin. Bacterial strains were incubated in polystyrene plates precoated with bovine submaxillary mucin for 90 minutes at 37°C. Plates were then washed three times with PBS and treated with 0.1% Triton X-100/PBS, and adherent bacteria were enumerated by dilution and plating. One-way ANOVA was performed on log-transformed data followed by Bonferroni’s test for multiple comparisons. Significance indicated as compared to 042 aafA (pBAD aafDA ). **** P ≤ 0.0001.

Article Snippet: Briefly, bacterial strains were grown to an OD 600 of 1.0 in LB at 37°C with shaking and then diluted 1:50 into DMEM high glucose with 2% arabinose and 100 μg/mL carbenicillin in 48-well polystyrene plates (Corning).

Techniques: Binding Assay, Incubation, Bacteria, Transformation Assay